Castro made the transfer to screen acting in 2005 in an appearance on the crime drama The Shield. She'd go on to make guest appearances in series like Glee, Little Britain USA, The Sarah Silverman Program, The Travelers, Two and a Half Men, Kroll Show, and Life in Pieces. Castro was a recurring cast member in the TV series "Go On" starring Matthew Perry in 2012-2013. In 2013, she was part of the main cast for the short lived sitcom Dads, which was executive produced by Seth MacFarlane. In film, Castro made appearances in Imagine That, Funny People, Our Family Wedding, Bad Ass, Seeking a Friend for the End of the World, The Book of Life, and In God's Time.

During her stint on Life in Pieces, Castro began feeling ill, and was eventually diagnosed with stomach cancer. She died from the disease on May 8, 2016.